A colorimeter is a device used in

colorimetry Colorimetry is "the science and technology used to quantify and describe physically the human color perception". It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color ...

that measures the

absorbance Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". Alternatively, for samples which scatter light, absorbance may be defined as "the negative lo ...

of particular

wavelength In physics, the wavelength is the spatial period of a periodic wave—the distance over which the wave's shape repeats. It is the distance between consecutive corresponding points of the same phase on the wave, such as two adjacent crests, tro ...

s of

light Light or visible light is electromagnetic radiation that can be perceived by the human eye. Visible light is usually defined as having wavelengths in the range of 400–700 nanometres (nm), corresponding to frequencies of 750–420 tera ...

by a specific

solution Solution may refer to: * Solution (chemistry), a mixture where one substance is dissolved in another * Solution (equation), in mathematics ** Numerical solution, in numerical analysis, approximate solutions within specified error bounds * Soluti ...

. It is commonly used to determine the

concentration In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture. Several types of mathematical description can be distinguished: '' mass concentration'', ''molar concentration'', ''number concentration'', an ...

of a known

solute In chemistry, a solution is a special type of homogeneous mixture composed of two or more substances. In such a mixture, a solute is a substance dissolved in another substance, known as a solvent. If the attractive forces between the solvent ...

in a given solution by the application of the

Beer–Lambert law The Beer–Lambert law, also known as Beer's law, the Lambert–Beer law, or the Beer–Lambert–Bouguer law relates the attenuation of light to the properties of the material through which the light is travelling. The law is commonly applied t ...

, which states that the concentration of a solute is proportional to the absorbance.

Construction

The essential parts of a colorimeter are: * a light source (often an ordinary low-voltage

filament lamp An incandescent light bulb, incandescent lamp or incandescent light globe is an electric light with a wire filament heated until it glows. The filament is enclosed in a glass bulb with a vacuum or inert gas to protect the filament from oxidat ...

); * an adjustable

aperture In optics, an aperture is a hole or an opening through which light travels. More specifically, the aperture and focal length of an optical system determine the cone angle of a bundle of rays that come to a focus in the image plane. An opt ...

; * a set of colored

filters Filter, filtering or filters may refer to: Science and technology Computing * Filter (higher-order function), in functional programming * Filter (software), a computer program to process a data stream * Filter (video), a software component tha ...

; * a

cuvette A cuvette ( French: cuvette = "little vessel") is a small tube-like container with straight sides and a circular or square cross section. It is sealed at one end, and made of a clear, transparent material such as plastic, glass, or fused quartz. ...

to hold the working solution; * a detector (usually a

photoresistor A photoresistor (also known as a photocell, or light-dependent resistor, LDR, or photo-conductive cell) is a passive component that decreases resistance with respect to receiving luminosity (light) on the component's sensitive surface. The elect ...

) to measure the transmitted light; * a meter to display the output from the detector. In addition, there may be: * a

voltage regulator A voltage regulator is a system designed to automatically maintain a constant voltage. A voltage regulator may use a simple feed-forward design or may include negative feedback. It may use an electromechanical mechanism, or electronic components ...

, to protect the instrument from fluctuations in

mains voltage Mains electricity or utility power, power grid, domestic power, and wall power, or in some parts of Canada as hydro, is a general-purpose alternating-current (AC) electric power supply. It is the form of electrical power that is delivered to ...

; * a second light path, cuvette and detector. This enables comparison between the working solution and a "blank", consisting of pure solvent, to improve accuracy. There are many commercialized colorimeters as well as

open source Open source is source code that is made freely available for possible modification and redistribution. Products include permission to use the source code, design documents, or content of the product. The open-source model is a decentralized sof ...

versions with construction documentation for education and for research.
Anzalone GC, Glover AG, Pearce JM. Open-Source Colorimeter. ''Sensors'' (2013); 13(4):5338-5346

Filters

Changeable Filter (optics), optics filters are used in the colorimeter to select the wavelength which the solute absorbs the most, in order to maximize accuracy. The usual wavelength range is from 400 to 700 nm. If it is necessary to operate in the

ultraviolet Ultraviolet (UV) is a form of electromagnetic radiation with wavelength from 10 nanometer, nm (with a corresponding frequency around 30 Hertz, PHz) to 400 nm (750 Hertz, THz), shorter than that of visible light, but longer than ...

range then some modifications to the colorimeter are needed. In modern colorimeters the filament lamp and filters may be replaced by several (light-emitting diode) of different colors.

Cuvettes

In a manual colorimeter the cuvettes are inserted and removed by hand. An automated colorimeter (as used in an

AutoAnalyzer The AutoAnalyzer is an automated analyzer using a flow technique called continuous flow analysis (CFA), or more correctly segmented flow analysis (SFA) first made by the Technicon Corporation. The instrument was invented in 1957 by Leonard ...

) is fitted with a flowcell through which solution flows continuously.

Output

The output from a colorimeter may be displayed by an analogue or digital meter and may be shown as

transmittance Transmittance of the surface of a material is its effectiveness in transmitting radiant energy. It is the fraction of incident electromagnetic power that is transmitted through a sample, in contrast to the transmission coefficient, which is th ...

linear Linearity is the property of a mathematical relationship (''function'') that can be graphically represented as a straight line. Linearity is closely related to '' proportionality''. Examples in physics include rectilinear motion, the linear r ...

scale from 0 to 100%) or as

logarithmic scale A logarithmic scale (or log scale) is a way of displaying numerical data over a very wide range of values in a compact way—typically the largest numbers in the data are hundreds or even thousands of times larger than the smallest numbers. Such a ...

from zero to infinity). The useful range of the absorbance scale is from 0 to 2 but it is desirable to keep within the range 0–1, because above 1 the results become unreliable due to scattering of light. In addition, the output may be sent to a

chart recorder A chart recorder is an electromechanical device that records an electrical or mechanical input trend onto a piece of paper (the chart). Chart recorders may record several inputs using different color pens and may record onto strip charts or circu ...

data logger A data logger (also datalogger or data recorder) is an electronic device that records data over time or about location either with a built-in instrument or sensor or via external instruments and sensors. Increasingly, but not entirely, they a ...

, or

computer A computer is a machine that can be programmed to Execution (computing), carry out sequences of arithmetic or logical operations (computation) automatically. Modern digital electronic computers can perform generic sets of operations known as C ...

Notes

References